Molecular Projects

The Green Plant BAC Library Project: Public resources for studying evolution, physiology and development - (Green Plant BAC Link ; Clemson Link) The goal of the Green Plant BAC project is to provide the resources, through the production of Bacterial Artificial Chromosome (BAC) libraries, for future research to elucidate the genetic basis for the transitions that mark the most fundamentally important steps in green plant evolution. This project helps scientists to answer the fundamental biological question: what is the genetic basis for the key innovations that enabled the green plants to diversify from aquatic unicellular or simple colonial life forms to life on land, an evolution that has led to the flowering plants as the dominant life form on earth today? This project provides the plant science community with the deep-coverage, large-insert DNA libraries that are required for obtaining complete gene sets whose roles in the genetic basis of these evolutionary innovations can then be examined in detail. It is very important that the plants chosen to provide the DNA for the construction of BAC libraries are part of long-term, ongoing genetics programs, are large enough to provide all of the required samples from one plant or clonal line, can be sampled repeatedly in the future, have a small genome size, and of course are of an evolutionarily important species. We also tried whenever possible to select species, and individual plants, that were also being used in other genome projects, such as the Floral Genome Project. Clone 108 in The University of Tennessee Tree Improvement Program (UT TIP) satisfied all of the requirements for the "GreenBAC" project. The material at the UTIP is the only known genetic defined stocks of Liriodendron trees and the contribution of clone 108 permitted this important project to proceed. The genetic material of Clone 108 has been immortalized in the form of a BAC library that is now available for use by the entire research community.

Principal and Co-Principal Investigators: Dina F. Mandoli (Directror) and Richard G. Olmstead of the University of Washington, Rod A. Wing of the University of Arizona, Jeffrey P. Tomkins of Clemson University, Jo Ann Banks of Purdue University, Claude W. dePamphilis and John E. Carlson of Penn State University. Other cooperators are listed at the web site.